Generator

Part:BBa_K511826:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Inducible N-Cadherin-2A-EYFP Generator (UAS-Gal4-N-Cadherin-2A-EYFP) MammoBlock Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 126
    Illegal SpeI site found at 2965
    Illegal PstI site found at 529
    Illegal PstI site found at 1630
    Illegal PstI site found at 3241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 126
    Illegal NheI site found at 154
    Illegal SpeI site found at 2965
    Illegal PstI site found at 529
    Illegal PstI site found at 1630
    Illegal PstI site found at 3241
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 126
    Illegal BamHI site found at 1162
    Illegal XhoI site found at 83
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 126
    Illegal SpeI site found at 2965
    Illegal PstI site found at 529
    Illegal PstI site found at 1630
    Illegal PstI site found at 3241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 126
    Illegal SpeI site found at 2965
    Illegal PstI site found at 529
    Illegal PstI site found at 1630
    Illegal PstI site found at 3241
    Illegal NgoMIV site found at 1481
    Illegal NgoMIV site found at 3007
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1516
    Illegal BsaI.rc site found at 2073


Design Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.


Source

Consult constituent components.

References